2008 Annual Meeting Abstracts
Catechin and Magnolol Ameliorate Oxidative Stress in Pancreatic Acinar Cells
Julie A. Alosi, MD, Debbie E. McDonald, BS, David W. McFadden, MD.
University of Vermont, Burlington, VT, USA.
Objective: Oxidative stress plays a role in the pathogenesis of acute pancreatitis. Catechin and magnolol, two naturally occurring molecules found in green tea and magnolia bark extract, have antioxidant activity. We hypothesized that catechin and magnolol would protect pancreatic acinar cells from peroxide mediated oxidative stress.
Design: Rat pancreatic acinar cells (AR42J) were cultured using standard techniques. Hydrogen peroxide (40, 80, and 120 μM) produced a graded oxidative injury. Cells were treated with catechin (10 and 40 μM) or magnolol (10 and 40 μM) pre- and post oxidative insult. Amylase levels were evaluated. Cell viability was determined using MTS tetrazolium assay. Apoptosis and necrosis were evaluated by Annexin-V flow cytometry and DNA fragmentation assay.
Results: The peroxide oxidative stress model produces a significant dose dependent decrease in cell viability noted at 24 and 48 hours. There is a trend of decreased amylase production by cells treated with catechin. Catechin and magnolol pretreatment produced a significant (p<0.05) increase in cell viability at 24 and 48 hours. Catechin post-treatment caused an even greater increase in cell viability (p<0.05). Catechin treated cells showed increased apoptosis and decreased necrosis, whereas magnolol treated cells had no change.
Conclusions: Catechin and magnolol improve in vitro cell survival in AR42J cells subjected to oxidative stress. Although there is an increase in apoptosis, decreased necrosis and its accompanying inflammatory cytokines likely contributes to the increase in cell viability seen with catechin-treated acinar cells. In addition, catechin’s effectiveness as a post-treatment highlights its potential for use in the treatment of acute pancreatitis.