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2010 Annual Meeting Abstracts

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T-cell immune responses after cryotherapy of breast cancer
Richard J Barth, Jr.1, *Dawn Fischer1, *Jacqueline Channon1, Wendy Wells1, *Gary Levine2, *Steve Poplack1
1Dartmouth, Lebanon, NH;2Hoag Memorial Presbyterian Hospital, Newport Beach, CA

Objective: Murine studies have demonstrated that cryotherapy can induce anti-tumor immune responses. Our objective was to determine whether cryotherapy of human breast cancer can induce an anti-tumor T-cell immune response.
Design: Prospective study.
Setting: Two academic tertiary care medical centers.
Patients: Twenty patients with infiltrating ductal carcinomas less than 1.5 cm in diameter.
Interventions: Under ultrasound guidance and local anesthesia the patient’s tumors underwent cryoablation with 2 freeze-thaw cycles. Blood samples were obtained prior to and 1 week after cryoablation. Tumors were resected 4 weeks after cryoablation.
Main outcome measures: Pentamer analysis for T-cells specific for breast cancer associated peptides Her-2 neu, CEA, Muc-1. Interferon γ ELISPOT analysis for these peptides plus NYBR-1, folate receptor α and MCF-7 breast cancer cell line lysate.
Results: Pathologic evaluation of all resected specimens showed no evidence of viable cancer cells in the cryoablation zone. T-cells specific for control peptides from the common viruses CMV and influenza were detected in patients with both pentamer and ELISPOT assays. Tumor peptide reactive T-cells were detected in patients prior to and after cryoablation. There was no increase in mean tumor peptide specific T-cell frequencies by pentamer analysis and there was no increase in mean interferon γ secreting, tumor peptide specific T-cells by ELISPOT analysis after cryoablation.
Conclusions: Cryoablation of small breast cancers in patients resulted in histologic evidence of tumor destruction, but did not induce a detectable anti-tumor T-cell immune response.


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