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2010 Annual Meeting Abstracts

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MicroRNA Expression Profiling in Malignant vs. Benign Human Thyroid Tumors
Hyunsuk Suh, *Susannah Orzell, *Samuel Kim, Jennifer E Rosen
Boston University, Boston, MA

Objective
• Collect and extract total RNA from fresh surgical human thyroid tumor specimens.
• Profile miRNAs for differential expression between benign vs. malignant samples using two different PCR array platforms.
• Perform hierarchical cluster analysis to identify signature miRNAs.

Design
• Total of 20 ex-vivo thyroid tumor (10 benign; 10 malignant) samples collected immediately post-op.
• miRNA expression levels profiled using RT² miRNA PCR Array System(376 miRNAs; SABiosciences Corporation, Frederick, MD) and V2 MicroRNA Expression Profiling Kit (1536 miRNAs; Illumina, San Diego, CA).

Setting
• Academic institution.
Patients
• Patients undergoing total or near total thyroidectomy with thyroid nodules.
Interventions
• None
Main Outcome Measures:
• miRNA expression profile based on either cDNA real-time PCR amplification (qRT-PCR; SABioscience) or beadchip cDNA hybridization method (Illumina).

Results
• Using the SABioscience kit, a set of four miRNAs (miR551b, miR146b, miR222, and miR375) were most differentially over-expressed (p=<0.05) and one miRNA (miR7) was under-expressed (p=.626) in malignant vs benign thyroid tumors. These findings were cross validated with the Illumina kit using the principle component analysis. Hierarchical cluster analysis of miRNA expression profile demonstrated that the two of the most statistically significant miRNAs (miR146 & 551) can completely classify benign vs. malignant tumors.

Conclusions
• Distinctive microRNA expression patterns exist between human thyroid carcinoma and adenoma.
• Only few miroRNAs are differentially expressed with statistical significance and may serve as a diagnostic marker.
• Differential expression profile of microRNAs may elucidate the mechanism of thyroid cancer tumorigenesis and biological relevance of respective microRNAs.


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